Use of Novel Triphenyltin (IV) Dithiocarbamate Compounds in Assessing the Cytotoxicity and Mode of Cell Death of Acute Lymphoblastic Leukemia Cell Lines, CCRF CEM (CCL-119)

Chemotherapy and radiotherapy are often used to treat Acute Lymphoblastic Leukemia (ALL). However, the resistance problem of treatment using current drug in ALL patients has decreased effectiveness such therapy. Therefore, a study needs be conducted identify potential new anticancer agents. Organotin (IV) dithiocarbamate, an organometallic compound, causes toxic effects on cancer cells vitro vivo. This examines cytotoxic mode cell death novel organotin dithiocarbamate compounds triphenyltin diisopropyldithiocarbamate (compound 1), diallyldithiocarbamate 2) diethyldithiocarbamate 3) acute lymphoblastic leukemia line, CCRF CEM (CCL-119). An MTT assay was for determination cytotoxicity by treating with 1-3 at highest concentration 10 µm 24 h. The induction 1, 2, 3 were identified Annexin V-FITC/PI assay. From results, all have substantial IC50 values between 0.18-0.20 μm. chemicals induced apoptosis CCL-119 cells, based mode. statistical analysis showed that only apoptotic 1-2 is significant compared negative controls. In conclusion, these high effect able cause via cells.